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KMID : 1134820110400050635
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 5 p.635 ~ p.641
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Suppressive Effects of Ethyl Acetate Fraction from Green Tea Seed Coats on the Production of Cell Adhesion Molecules and Inflammatory Mediators in Human Umbilical Vein Endothelial Cells
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Noh Kyung-hee
Kim Jong-Kyung
Song Young-Sun
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Abstract
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Anti-atherogenic effects in tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells (HUVEC) are involved with suppressed oxidative stress, cell adhesion molecules, and pro-inflammatory factors. The aim of this study was to determine whether green tea seed coat ethyl acetate fraction (GTSCE) could modulate cell adhesion molecules and inflammatory mediators in HUVEC stimulated with TNF-α. Nitric oxide (NO) production was significantly increased in TNF-α-stimulated HUVEC compared to TNF-α only treated cells. The NO that is produced by endothelial nitric oxide synthase dilates blood vessels and has protective effects against platelet and leucocyte adhesion. GTSCE at 25, 50, 75, and 100 ㎍/mL significantly (p＜0.05) reduced TNF-α production. GTSCE significantly (p＜0.05) inhibited soluble vascular cell adhesion molecule-1 level, in a dose-dependent manner. Monocyte chemoattractant protein-1 level was also significantly (p＜0.05) inhibited by GTSCE treatment at 75 ㎍/mL compared to the TNF-α-only treated group. Total antioxidant capacity by GTSCE was significantly (p＜0.05) enhanced compared to the TNF-α-only treated group. These results suggest that GTSCE can inhibit the production of cell adhesion molecules and inflammatory mediators and could be used as a candidate bioactive material to prevent the development of atherosclerosis.
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KEYWORD
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HUVEC, green tea seed coat, pro-inflammatory mediators, cell adhesion molecules
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